On the dynamics of HLA-A2 antigen-antibody complexes on the cell membrane of human peripheral blood lymphocytes. A biochemical and electron microscopical autoradiographic study

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Abstract
Some dynamic aspects of membrane bound immune complexes of HLA antigens and antibodies are reported. Radiolabeled HLA antibodies were used to sensitize peripheral blood lymphocytes. Upon in vitro incubation of these cells, 2 different events were detected. Immune complexes and a limited amount of free antibody were shed into the culture supernate; from the immune complexes specific antibodies could be regenerated. Immune complexes were also internalized by means of endocytosis as visualized by EM autoradiography. Antibodies were found above dense bodies (lysosomes) and above multivesicular bodies. Results are discussed in relation to the biology of membrane bound HLA molecules.