Identification and Typing of Viruses and Detection of Antiviral Antibodies by Counterimmunoelectrophoresis

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Abstract
20 virus types and subtypes belonging to several viral groups and their respective antisera were tested by counterimmunoelectrophoresis (CIEP). Group-, type- and/or subtype-specific precipitation lines were formed provided the viral antigens and antisera were sufficiently concentrated and potent. Although the CIEP test itself is more rapid (less than 3 h) and simple than other commonly used tests for virus identification, such as complement fixation, it requires concentration of the antigen to a considerable degree, especially in the case of viruses with little antigenic mass (e.g. polioviruses).

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