Lymphoblastoid cell lines and burkitt‐lymphoma‐derivee cell lines differ in the expression of a second epstein‐barr virus encoded nuclear antigen

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Abstract
Twenty‐seven Epstein‐Barr virus (EBV)‐transformed lymphoblastoid cell lines and 27 EBV‐carrying Bur‐kitt‐lymphoma‐derived lines were analyzed for expression of the second EBV‐encoded nuclear antigen (EBNA‐2) by immunoblotting and anticomple‐ment immunofluorescence with EBNA‐2‐specific sera. While all lymphoblastoid cell lines expressed EBNA‐2, only 10 of the 27 BL lines were EBNA‐2‐positive. Comparison of the EBNA‐2 coding Bam HI W‐Y‐ and H‐fragments of EBV‐DNA in the different cell lines by restriction enzyme analysis suggests that EBNA‐2 negativity is due either to sequence diversity or to a deletion in the BamHI WYH region.
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