Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in Saccharomyces cerevisiae
- 31 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 3 (4) , 439-444
- https://doi.org/10.1093/carcin/3.4.439
Abstract
Ethyl methanesulfonate (EMS)-induced reversion of 2 sites which revert preferentially by GC to AT transitions, cycl-131 and cycl-115, was examined in radiation sensitive, rad, mutants of yeast [S. cerevisiae] belonging to the rad52 epistasis group. The rad50, rad51, rad52, rad54 and rad56 mutants showed reduced reversion of both tester sites when stationary phase diploid cells were treated with EMS. No correlation was found between EMS-induced reversion and EMS-induced homologous mitotic intragenic recombination. Survival of rad6 rad52 double mutants following EMS treatment indicates that there is 1 epistasis group for the repair of EMS-induced lethal damage in yeast. A model involving misrepair mutagenesis of specific lesions is proposed to account for the experimental results.This publication has 23 references indexed in Scilit:
- Pathways of mutagenesis and repair in Escherichia coli exposed to low levels of simple alkylating agentsJournal of Bacteriology, 1978
- Recombinational repair of alkylation lesions in phage T4Molecular Genetics and Genomics, 1978
- The influence of defects in excision and error prone repair on spontaneous and induced mitotic recombination and mutation in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1978
- Genetic studies of the lac repressorJournal of Molecular Biology, 1977
- ISOLATION AND CHARACTERIZATION OF MMS-SENSITIVE MUTANTS OF SACCHAROMYCES CEREVISIAEGenetics, 1977
- Alkaline sucrose sedimentation studies of MMS-induced DNA single-strand breakage and rejoining in the wild type and in UV-sensitive mutants of Saccharomyces cerevisiaeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- EFFECT OF GENES CONTROLLING RADIATION SENSITIVITY ON CHEMICALLY INDUCED MUTATIONS IN SACCHAROMYCES CEREVISIAEGenetics, 1976
- UV MUTAGENESIS IN RADIATION-SENSITIVE STRAINS OF YEASTGenetics, 1976
- The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic controlMolecular Genetics and Genomics, 1976
- Respiration-deficient mutants of yeastBiochimica et Biophysica Acta (BBA) - General Subjects, 1964