Quantitative Determination of Glycosphingolipids Illustrated by Using Erythrocyte Membranes of Various Mammalian Species
- 1 September 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 79 (1) , 265-267
- https://doi.org/10.1111/j.1432-1033.1977.tb11805.x
Abstract
A procedure for the quantitative determination of glycosphingolipids is described, involving extraction of total lipids, fractionation on a column, acid cleavage and photometry of a complex formed between sphingosine and methyl orange. The level of glycosphingolipid sphigosine of erythrocyte membranes of a variety of mammals [human, llama, cat, sheep, bovine] ranges from 59.1 (rat) to 410.1 (pig) nmol/ml packed cells.This publication has 10 references indexed in Scilit:
- Unique properties of the camel erythrocyte membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Studies on the Attachment of the Bovine J Blood‐Group Substance at the Erythrocyte MembraneEuropean Journal of Biochemistry, 1971
- Quantitative isolation of total glycosphingolipids from animal cellsJournal of Lipid Research, 1971
- Quantitative determination of the neutral glycosyl ceramides in human bloodJournal of Lipid Research, 1967
- The Use of Sephadex for the Removal of Nonlipid Contaminants from Lipid Extracts*Biochemistry, 1963
- Thin-layer chromatographic separation of sphingosine and related basesJournal of Lipid Research, 1963
- A spectrophotometric determination of sphingosineJournal of Lipid Research, 1962
- The Thiobarbituric Acid Assay of Sialic AcidsJournal of Biological Chemistry, 1959
- Phospholipids. 4. On the composition of hen's egg phospholipidsBiochemical Journal, 1957
- Investigation on Lipid Separation Methods: Separation of Phospholipids from Neutral Fat and Fatty AcidsActa Physiologica Scandinavica, 1952