Phospholipids. 4. On the composition of hen's egg phospholipids

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Abstract
The extraction and purification of hen''s egg phospholipids were examined, and a mild procedure yielding 97% of the total material was devised. The crude phospholipid was separated chromatographically on alumina into choline-containing and non-choline-containing fractions, with quantitative recovery of both. By further separation on silicic acid the 2 major constituents were recovered in a practically pure condition, and the minor constituents concentrated sufficiently to permit identification and estimation. The egg phospholipid examined contained phosphatidylcholine 73.0, lysophosphatidyl-choline 5.8, sphingomyelin 2.5, phosphatidylethanolamine 15.0, lysophosphatidylethanolamine 2.1 and inositol phospholipid 0.6, all as moles %. The plasmalogen content of the whole phospholipid was 0.9%, most of which was present in the lecithin fraction. The phosphatidylethanolamine fraction contained a small proportion (0.2% of the total phospholipid) of amino acid-containing phospholipid.