FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements
- 27 March 2000
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 88 (1-2) , 114-118
- https://doi.org/10.1159/000015502
Abstract
We combined the techniques of fluorescence in situ hybridization (FISH) and chromosomal microdissection in one experiment (FISH-MD). This novel method permits rapid identification of the composition, origin, and breakpoints of rearranged chromosomes. Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints.Keywords
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