Effect of Sodium Chloride and pH on Enterotoxin B Production

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Abstract
The growth and production of enterotoxin-B by Staphyloccus aureua strain S-6 in Brain Heart Infusion broth with 2 to 16% sodium chloride and an initial pH of 5.1 to 6.9 was studied during a 10-day incubation period at 37 C. Growth was good at pH 6.9 and with a 16% concentratio of salt, but no cells survived after 10 days of incubation at pH 5.1 and with a 16% concentration of salt. With gel diffusion technique, enterotoxin-B was detected in broth with pH 6.9 and up to 10% salt or pH 5.1 and up to 4% salt. Growth and enterotoxin production were better when pH was increased and salt concentration was decreased. The dependence of toxin production on the interaction of these two factors was demonstrated.