Differential sensitivity to guanine nucleotides of basal and insulin‐stimulated glucose transporter activity reconstituted from adipocyte membrane fractions
- 25 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (2) , 259-264
- https://doi.org/10.1016/0014-5793(89)81102-0
Abstract
The effects of GTPγS on glucose transport activity reconstituted from adipocyte membrane fractions were studied in order to test the hypothesis that intrinsic activity changes of the insulin‐sensitive glucose transporter may be mediated by guanine nucleotide‐dependent mechanisms. GTPγS and GTP inhibited reconstituted glucose transport activity by 50% in membrane fractions from insulin‐treated cells in a concentration‐dependent manner; no inhibitory effect was observed in membrane fractions obtained from basal cells. GDP, GMP and guanosine were less effective than GTP, whereas the adenine nucleotides ATPγS and AMP failed to reduce the reconstituted transport activity. The data indicate that guanine nucleotides may modulate the activity of the adipocyte glucose transporter. Since the effect is dependent on treatment of cells with insulin, the hormone appears to induce a specific functional alteration of the glucose transporter.Keywords
This publication has 16 references indexed in Scilit:
- Adipocyte plasma membranes contain two Gi subtypes but are devoid of GoFEBS Letters, 1988
- Qualitative and quantitative comparison of glucose transport activity and glucose transporter concentration in plasma membranes from basal and insulin-stimulated rat adipose cellsBiochemical Journal, 1987
- Insulin-stimulated glucose transport in rat adipose cells. Modulation of transporter intrinsic activity by isoproterenol and adenosine.Journal of Biological Chemistry, 1986
- Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.Proceedings of the National Academy of Sciences, 1986
- Insulin inhibits the cholera-toxin-catalysed ribosylation of a Mr-25000 protein in rat liver plasma membranesBiochemical Journal, 1985
- Insulin-stimulated translocation of glucose transporters in the isolated rat adipose cells: Characterization of subcellular fractionsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1983
- Insulin exerts actions through a distinct species of guanine nucleotide regulatory protein: inhibition of adenylate cyclaseBiochemical Journal, 1983
- An improved method of reconstitution of adipocyte glucose transport activityAnalytical Biochemistry, 1982
- Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. Apparent translocation of intracellular transport systems to the plasma membrane.Journal of Biological Chemistry, 1980
- Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site.Proceedings of the National Academy of Sciences, 1980