Changes in the Lipid and Fatty Acid Composition of Vicia faba Mesophyll Protoplasts Induced by Isolation

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Abstract
The lipid and fatty acid compositions of mesophyll protoplasts of Broad bean (Vicia faba) have been analysed by gas chromatography. The results indicate that protoplast isolation triggered the rapid hydrolysis of several membrane lipids, notably phosphatidylcholine and monogalactosyldiacylglycerol. The decrease of these lipids was compensated for by an increase in the amount of the neutral lipid fraction. Analyses of the fatty acid compositions suggest that phosphatidylcholine from the microsomes and monogalactosyldiacylglycerol from the chloroplast were degraded to diacylglycerol and free fatty acids and used for the biosynthesis of triacylglycerols.