Insulin but not phorbol ester treatment increases phosphorylation of vinculin by protein kinase C in BC3H‐1 myocytes
- 6 April 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 214 (1) , 122-126
- https://doi.org/10.1016/0014-5793(87)80025-x
Abstract
Insulin was found to increase protein kinase C activity in BC3H-1 myocytes as determined by in vitro phosphorylation of both a lysine-rich histone fraction (histone III-S) and vinculin. TPA treatment for 20 min or 18 h provoked an apparent loss of histone-directed but not vinculin-directed phosphorylation by cytosolic C-kinase. Thus, chronic TPA-induced ‘desensitization’ or ‘depletion’ of cellular protein kinase C is more apparent than real, and is not a valid means for evaluating the role of C-kinase in hormone action.Keywords
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