Hepatocarcinogenic heterocyclic aromatic amines that induce cytochrome P-448 isozymes, mainly cytochrome P-448H (P-450IA2), responsible for mutagenic activation of the carcinogens in rat liver

Publisher Website
Google Scholar
Abstract
Male F344 rats were treated with hepatocarcinogenic heterocydic aromatic amines such as amino acid-and protein-pyrolysate components (Trp P-1, Trp P-2, Glu P-1, Glu P-2, AαC, MeAαC, IQ and MeIQx) and changes in microsomal cytochrome P-450 isozymes in the livers were examined by means of immuno-Western blotting using anti-rat cytochrome P-450 monoclonal antibodies. The results suggested that all chemicals tested induce cytochrome P-448 isozymes, particularly cytochrome P-448H (P-450IA2), which efficiently mediate mutagenic activation of the carcinogens. This was substantiated by the enzymatic analyses with the substrates showing different characters to rat cytochrome P-450 isozyme-mediated mutagenesis.

This publication has 13 references indexed in Scilit: