Inhibitory Effects of Mizolastine on Ultraviolet B‐Induced Leukotriene B4 Production and 5‐Lipoxygenase Expression in Normal Human Dermal Fibroblasts In Vitro

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Abstract
Ultraviolet B (UVB) irradiation exerts hazardous effects such as acute photodamage, skin cancer and photoaging. In this study we evaluated the protective effects of a nonsedative histamine H1‐receptor antagonist, mizolastine, on UVB‐exposed skin dermal fibroblasts. Therefore, primary human skin fibroblasts were incubated with mizolastine or dexamethasone after 100 mJ/cm2 UVB irradiation. Leukotriene B4 (LTB4) in fibroblast supernatants was detected with enzyme immunoassays, expression of 5‐lipoxygenase (5‐LOX) messenger RNA (mRNA) in skin fibroblasts was examined by reverse transcriptase‐polymerase chain reaction and expression of 5‐LOX protein was measured by immune blotting and immunofluorescent staining with rabbit anti‐human 5‐LOX antibody. It was found that 0.01 mM mizolastine inhibited UVB‐induced LTB4 production from skin fibroblasts at 12, 24 and 36 h. Meanwhile, mizolastine down‐regulated 5‐LOX mRNA expression and inhibited 5‐LOX translocation from nucleus to cytoplasm in fibroblasts. On the basis of these findings, we propose that mizolastine might play a protective role in the pathogenesis of UV radiation‐induced acute photodamage of the skin.

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