Isolation of a new gene (SW A2) encoding an α‐amylase from Schwanniomyces occidentalis and its expression in Saccharomyces cerevisiae

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Abstract
A new gene (SW A2) encoding a secretory α‐amylase activity from Schwanniomyces occidentalis has been cloned from this year and then expressed in Saccharomyces cerevisiae. Both Sw. occidentalis and a transformant of S. cerevisiae incorporating SW A2 contain a transcript of 2.1 kb which hybridizes to DNA carrying the SW A2 gene. This indicates that the transcript is a product of the SW A2 gene. Transcription of the SW A2 gene seems to be regulated in both Sw. occidentalis and S. cerevisiae. Furthermore, the secretion of α‐amylase activity is drastically expressed by glucose in both Sw. occidentalis and a transformant of S. cerevisiae containing SW A2.