Isolation and expression in Saccharomyces cerevisiae of a gene encoding an α‐amylase from Schwanniomyces castellii
- 25 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (2) , 455-459
- https://doi.org/10.1016/0014-5793(89)81144-5
Abstract
A gene (SWA1) encoding an α‐amylase activity from Schwanniomyces castellii has been cloned and expressed, via yeast cloning vector YEp13, in Saccharomyces cerevisiae. By using a riboprobe which is internal to the SWA1 gene, a 1.55 kb transcript was detected in the poly(A)+ RNA from both Sw. castellii and a S. cerevisiae clone harboring the SWA1 gene. This transcript should, therefore, correspond to the SWA1 gene. In addition, the DNA strand determining the α‐amylase activity has been defined. Transcription of the SWA1 gene appears to be highly regulated in Sw. castellii, whereas it is constitutive in the S. cerevisiae harboring this gene.Keywords
This publication has 19 references indexed in Scilit:
- Cloning of the orotidine 5?-phosphate decarboxylase (ODC) gene of Schwanniomyces occidentalis by complementation of the ura3 mutation in S. cerevisiaeCurrent Genetics, 1988
- Nucleotide sequence of the α‐amylase gene (ALP1) in the yeast Saccharomycopsis fibuligeraFEBS Letters, 1987
- Transformation of yeast spheroplasts without cell fusionAnalytical Biochemistry, 1987
- Schwanniomyces: A Potential Superyeast?Critical Reviews in Biotechnology, 1987
- The utilization of starch by yeastsYeast, 1986
- Identification and physical characterization of yeast glucoamylase structural genesMolecular Genetics and Genomics, 1986
- Cloning of theSTA2 andSGAgenes encoding glucoamylases in yeasts and regulation of their expression by theSTA10 gene ofSaccharomyces cerevisiaeNucleic Acids Research, 1986
- Effect of medium composition on excretion and biosynthesis of the amylases of Schwanniomyces castelliiApplied Microbiology and Biotechnology, 1980
- Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 geneGene, 1979
- Genetic studies of ability to ferment starch in Saccharomyces: Gene polymorphismMolecular Genetics and Genomics, 1978