Inhibition of vaccinia mRNA methylation by 2',5'-linked oligo(adenylic acid) triphosphate.
- 1 April 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (4) , 2221-2224
- https://doi.org/10.1073/pnas.78.4.2221
Abstract
Extracts of interferon-treated (mouse neoplastic fibroblast L929) cells synthesize unique 2'',5''-linked oligo(adenylic acid) 5''-phosphates in the presence of ATP and double-stranded RNA. 2'',5''-Linked oligo(adenylic acid) 5''-triphosphate inhibits protein synthesis at nanomolar concentrations by activating RNase. Oligo(adenylic acid) 5''-monophosphate and 5''-triphosphate are evidently potent inhibitors of vaccinia mRNA methylation in vitro. Both the methylation of the 5''-terminal guanine at the 7 position and the 2''-O-ribose methylation of the penultimate nucleoside are inhibited. Such inhibition of mRNA methylation is not due to degradation of the mRNA. Inhibition of the requisite modification of the 5'' terminus of mRNA by 2'',5''-linked oligo(adenylic acids) may be a mechanism of interferon action against DNA and RNA viruses in which mRNA derived from them are capped.This publication has 45 references indexed in Scilit:
- Accumulation of methyl-deficient rat liver messenger ribonucleic acid on ethionine administrationBiochemistry, 1980
- Natural occurrence of 2-5A in interferon-treated EMC virus-infected L cellsNature, 1979
- Activation of a nuclease by pppA2′p5′ A2′p5′ A in intact cellsFEBS Letters, 1979
- Interferon-associated, dsRNA-dependent enzyme activities in a mutant 3T6 cell engaged in the semiconstitutive synthesis of interferonCell, 1978
- Interferon, Double‐Stranded RNA and RNA DegradationEuropean Journal of Biochemistry, 1977
- Specific phosphorylation in vitro of a protein associated with ribosomes of interferon‐treated mouse L cellsFEBS Letters, 1976
- Inhibition of reovirus messenger RNA methylation in extracts of interferon-treated ehrlich ascites tumor cellsBiochemical and Biophysical Research Communications, 1975
- The Effect of Interferon on the Formation of Virus Polyribosomes in L Cells Infected with Vaccinia VirusJournal of General Virology, 1975
- Increased sensitivity of cell-free protein synthesis to double-stranded RNA after interferon treatmentNature, 1974
- Interferon inhibits Viral Protein Synthesis in L Cells infected with Vaccinia VirusNature, 1972