The Allergenic Activity and Stability of Purified Allergens from the Pollen of Common Rye Grass (Lolium perenne)

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Abstract
Allergenic activity has been found in 2 discrete groups of isoallergens from rye grass pollen. These 2 groups have previously been shown to be quite distinct physically, chemically and immunologically. Different allergic individuals showed different degrees of skin reaction towards the 2 allergenic groups. A few individuals were also allergic towards further allergens which have not yet been characterised. No detectable loss of skin activity was observed after digestion of a homogeneous group I allergen with the carbohydrases [beta]-galactosidase and cellulase, both of which were shown to release carbohydrate fragments from this allergen. This result suggests that a carbohydrate moiety is inessential for allergenlc activity in the group I rye allergens, especially when considered together with earlier experimental evidence supporting this view. Rye allergens are very heat stable and retain full allergenic activity over a wide pH range. Allergenic activity ofthe major rye allergens couldnot be inhibited by a large excess ofthe non-allergenic dialysable material obtainedfrom whole pollen extracts. This results suggests that no haptenic material'' is removedby dialysis.

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