Action of Rifamycin Derivatives on RNA Polymerase of Human Leukemic Lymphocytes
- 1 July 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (7) , 2072-2076
- https://doi.org/10.1073/pnas.70.7.2072
Abstract
The mechanism of inhibition of human RNA polymerase by four rifamycin derivatives was investigated. Derivative AF/013 (3-formyl rifamycin SV:O-n-octyloxime) with strong hydrophobic side chains prevents the polymerase from binding to DNA and also affects the size of RNA synthesized. Derivative PR/19 (3'-acetyl-1'-benzyl-2'-methylpyrrolo[3,2-c]-4-desoxy-rifamycin SV) only affects RNA synthesis when RNA polymerase has been previously incubated with the drug or when the reaction was performed at high salt concentration [0.14 M (NH(4))(2)-SO(4)]. Our results suggest that these drugs exert their inhibitory actions by binding to the enzyme instead of DNA.Keywords
This publication has 13 references indexed in Scilit:
- Characterization of T7 specific RNA polymerase. III. Inhibition by derivatives of rifamycin SVBiochemical and Biophysical Research Communications, 1972
- Animal DNA‐Dependent RNA PolymerasesEuropean Journal of Biochemistry, 1972
- Molecular Structures of DNA-Dependent RNA Polymerases (II) from Calf Thymus and Rat LiverProceedings of the National Academy of Sciences, 1971
- Actions of the rifamycins.1971
- RNA POLYMERASEAnnual Review of Biochemistry, 1971
- Specific Inhibition of Nuclear RNA Polymerase II by α-AmanitinScience, 1970
- Multiple Forms of DNA-dependent RNA Polymerase in Eukaryotic OrganismsNature, 1969
- Mode of action of rifamycin on the RNA polymerase reactionBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- INHIBITION OF DNA-DEPENDENT RNA SYNTHESIS BY RIFAMYCINSThe Journal of Antibiotics, 1968
- Base Specificity in the Interaction of Polynucleotides with Antibiotic DrugsScience, 1965