A Convenient Void Volume Marker for Several Chiral HPLC Columns
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 14 (1) , 1-8
- https://doi.org/10.1080/01483919108049593
Abstract
Comparison of a number of potential void volume markers under normal phase conditions shows 1,3,5-Tri-t-butylbenzene (TTBB) to be essentially unretained on three commercially available chiral stationary phases (CSPs). Elution times observed for TTBB agree well with void times determined by the method of minor disturbance, or with the pycnometrically determined total mobile phase volume (Vm). Therefore TTBB is recommended for use as a void volume marker for normal phase separations with these CSPs.Keywords
This publication has 17 references indexed in Scilit:
- Experimental and theoretical model of refractive index artifacts in absorbance detectionJournal of Chromatography A, 1988
- Solute Retention in Column Liquid Chromatography. XI. Exposition of the Minor-Disturbance Method of Measurement of the Void VolumeJournal of Liquid Chromatography, 1988
- Solute Retention in Column Liquid Chromatography: IX. Comparison of Methods of Determination of the Void Volume in Liquid-Liquid ChromatographyJournal of Liquid Chromatography, 1987
- A Review of methods for the Determination of Hold-up Volume in Modern Liquid ChromatographyJournal of Liquid Chromatography, 1986
- Movement of components in reversed-phase chromatography : I. Mobile phase space with multi-component eluentsJournal of Chromatography A, 1983
- Comparison of methods used for the determination of void volume in reversed-phase liquid chromatographyAnalytical Chemistry, 1982
- Chiral HPLC (high-performance liquid chromatographic) stationary phases. 4. Separation of the enantiomers of bi-.beta. naphthols and analogsThe Journal of Organic Chemistry, 1981
- Distribution equilibria of solvent components in reversed-phase liquid chromatographic columns and relationship with the mobile phase volumeJournal of Chromatography A, 1981
- Distribution phenomena of mobile-phase components and determination of dead volume in reversed-phase liquid chromatographyAnalytical Chemistry, 1980
- Movement and band spreading of unsorbed solutes in liquid chromatographyJournal of Chromatography A, 1976