The quantitative estimation of 3:4-benzpyrene in whole animals, their tissues and excreta

Publisher Website
Google Scholar
Abstract
A method is described for the quantitative estimation of 3:4-benzpyrene in whole mice, separate tissues, tissue fluids and excreta. It is based on the "wet" method of extraction and spectrographic analysis of the fluorescence of the extract, originally described by Berenblum & Kendal (1936) for the estimation of 1:2:5:6:-dibenzanthracene. The most important changes in the present method are concerned with the purification of the extract for the removal of interfering substances. These result in a considerable simplification of the procedure and a greater sensitivity. Limit of sensitivity: about 0.05 [gamma] per mouse; accuracy: about [plus or minus]20% over a range of 1-10,000 [gamma] per mouse.