IMMUNOLOGICAL AND SEROLOGICAL DATA CONCERNING HUMAN HISTOCOMPATIBILITY ANTIGENS (HLA) AND ALPHA-1-MICROGLOBULIN CO-PURIFIED FROM URINE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 31  (8) , 233-236
Abstract
Serologically active glycoproteins (HLA) purified from urine contained only about 4% HLA antigens copurified with .alpha.1-microglobulin. The immunization of rabbits with the serologically active product gave rise to an antiserum containing 2 kinds of antibodies: anti-HLA-A9 and anti-.alpha.1-microglobulin. The anti-HLA-A9 antibodies were detected by the lymphocytotoxicity and indirect immunofluorescence techniques against peripheral blood lymphocytes and cultured lymphoid cell lines. Anti-.alpha.1-microglobulin antibodies were detected by immunoprecipitation in gel. Pure .alpha.1-microglobulin gave rise to an antiserum directed only against .alpha.1-microglobulin. This antiserum did not react with peripheral blood lymphocytes and cultured lymphoid cell lines.