Autoradiographic Plaques for the Detection of Antibody Formation to Soluble Proteins by Single Cells
- 18 May 1967
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 156 (3777) , 964-966
- https://doi.org/10.1126/science.156.3777.964
Abstract
Single cells that synthesize antibody to soluble proteins can be detected and counted by an autoradiographic procedure. The method is based on the specific binding of I131-labeled antigen to the antidogy which has been produced by and has diffused around cells in an agarose medium and which has been precipitated by an antibody to gamma globulin. The antigen-binding zones appear as dark spots on high-speed x-ray film exposed to the dried agarose preparations.This publication has 10 references indexed in Scilit:
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