Specificity of anti-nucleoside antibodies
- 1 July 1974
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 141 (1) , 313-315
- https://doi.org/10.1042/bj1410313
Abstract
The method of conjugation of a nucleoside or related compound to a carrier protein may have a significant effect on the specificity of the antibodies elicited. It is demonstrated, by means of the membrane-filtration assay, that anti-isopentenyladenosine antibodies produced by the ‘periodate procedure’ are much more reactive with the periodate-oxidized form of the nucleoside than with the parent compound. In addition, the simplicity and specificity of the assay used suggests its use as a sensitive radioimmunoassay for this multifunctional nucleoside.Keywords
This publication has 11 references indexed in Scilit:
- Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purificationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding techniqueBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Nucleic acid related compounds. 6. Sugar-modified N6-(3-methyl-2-butenyl)adenosine derivatives, N6-benzyl analogs, and cytokinin-related nucleosides containing sulfur or formycinBiochemistry, 1973
- Modified nucleosides with antineoplastic activity as inhibitors of an enzyme containing adenosine within its coenzyme.1973
isopentenyl)adenosine : The regulatory effects of a cytokinin and modified nucleoside from tRNA on human lymphocytesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972- N6-(δ2-Isopentenyl)adenosine: Chemical Reactions, Biosynthesis, Metabolism, and Significance to the Structure and Function of †RNAProgress in Nucleic Acid Research and Molecular Biology, 1970
- Analysis of nucleic acid derivatives at the subnanomole levelAnalytical Biochemistry, 1969
- The Preparation of Nucleotide-Protein Conjugates: Carbodiimides as Coupling AgentsThe Journal of Immunology, 1966
- ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNAProceedings of the National Academy of Sciences, 1964
- The Reaction of Methylamine with Periodate-Oxidized Adenosine 5'-PhosphateBiochemistry, 1963