ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNA
- 1 July 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (1) , 68-74
- https://doi.org/10.1073/pnas.52.1.68
Abstract
A general method for the preparation of conjugates of proteins with nucleosides and nucleotides is described. The conjugates were antigenic in the rabbit and elicited the formation of antibodies specific for the purine or pyrimidine nucleotide or nucleoside used as the determinant group. Antisera to Guan-BSA, Cyt-BSA, UMP-BSA, and AMP-BSA were found by complement fixation to react with thermally denatured DNA. No reaction with RNA could be demonstrated. The use of these antibodies as tools for the elucidation of the structure and function of DNA is discussed.Keywords
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