STIMULATING CAPACITY OF FRESH AND CULTURED HUMAN LEUKEMIC LYMPHOID AND MYELOID CELLS IN ONE-WAY MIXED LYMPHOCYTE-REACTION

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 33  (4) , 543-551
Abstract
Fresh normal peripheral blood B [bone marrow-derived] lymphocytes possess a strong stimulating capacity, while fresh thymus cells or fresh peripheral T [thymus-derived] lymphocytes possess a weak, but significant stimulating capacity on allogeneic lymphocytes in one-way mixed lymphocyte reaction. Fresh leukemic T lymphoid cells from patients with T-cell ALL [acute lymphocytic leukemia] or T-cell CLL [chronic lymphocytic leukemia] exert little or no stimulation on allogeneic lymphocytes. Fresh leukemic B lymphoid cells from patients with B-cell CLL or B-cell HCL [hairy cell leukemia] exert a lesser stimulation on allogeneic lymphocytes as compared to that of normal B lymphocytes. Leukemic myeloblasts from patients with AML [acute myelogenous leukemia] or pH1(+) CML[chronic myelogenous leukemia]-BP exert significantly higher stimulation than leukemic lymphoid cells in one-way mixed lymphocyte reaction (P < 0.05). Cultured leukemic T lymphoid [human] cells (MOLT-4) possess no stimulating capacity, cultured leukemic B lymphoid [human] cells (BALM-2) possess a moderate degree of stimulating capacity and cultured leukemic, possibly myeloid, [human] cells (NALM-1 and K562) possess vigorous stimulation on allogeneic lymphocytes. The stimulating capacity of NALM-1 or K562 cells is significantly higher than that of BALM-2 cells (P < 0.01 or P < 0.05, respectively) and that of MOLT-4 cells (P < 0.001). The stimulating capacity of leukemic T or B lymphoid cells may be completely or partially lost during the process of leukemogenesis. It is not known whether the stimulating capacity of leukemic myeloblasts, which is very strong on allogeneic lymphocytes, may be modified during the process of leukemogenesis.