DEOXYRIBONUCLEASE IV: A NEW EXONUCLEASE FROM MAMMALIAN TISSUES
- 1 February 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 62 (2) , 597-603
- https://doi.org/10.1073/pnas.62.2.597
Abstract
An exonuclease which specifically degrades double-standard DNA has been isolated from rabbit tissues. The enzyme has an approximate molecular weight of 42,000, requires a divalent metal ion as cofactor, and attacks DNA at the 5′-terminal ends, thereby liberating 5′-mononucleotides. It degrades several synthetic polydeoxynucleotides of single repeating base sequences more rapidly than DNA from natural sources. The specificity of this mammalian enzyme resembles that of several microbial enzymes (phage λ exonuclease and DNA polymerase) which appear to be required for repair and recombination of DNA.Keywords
This publication has 24 references indexed in Scilit:
- Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductasesPublished by Elsevier ,2003
- Enzymatic breakage and joining of deoxyribonucleic acid. V. End group labeling and analysis of deoxyribonucleic acid containing single straned breaks.1968
- Exonuclease VI, a new nuclease activity associated with E. coli DNA polymerase.Proceedings of the National Academy of Sciences, 1968
- Purification from Calf Spleen of Two Inhibitors of Deoxyribonuclease I. Physical and Chemical Characterization of the Inhibitor II*Biochemistry, 1967
- The exonucleolytic nature of an alkaline deoxyribonuclease of mammary tumors of C3H miceBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- The nature of poxvirus-induced deoxyribonucleasesBiochemical and Biophysical Research Communications, 1966
- The purification from rat liver of a nuclease hydrolysing ribonucleic acid and deoxyribonucleic acidBiochemical Journal, 1966
- A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activityBiochemical Journal, 1964
- A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein MixturesJournal of Biological Chemistry, 1961
- Presence of two different desoxyribonucleodepolymerases in veal kidneyBiochimica et Biophysica Acta, 1953