LACTOPEROXIDASE AND THIOCYANATE PROTECT CULTURED MAMMALIAN-CELLS AGAINST HYDROGEN-PEROXIDE TOXICITY

  • 1 January 1983
    • journal article
    • research article
    • Vol. 61  (5) , 268-274
Abstract
The toxicity of various combinations of lactoperoxidase-thiocyanate H2O2 was tested on a cell line of human epidermoid carcinoma of the cervix of (HeLa), a Chinese hamster ovary cell line (CHO) and human gingival fibroblasts. The capacity of the cells to proliferate, to exclude trypan blue and to produce acids from glucose was evaluated. There was no significant difference between the 3 cell types in their reactions to the various test combinations. A 1 h exposure of the cells to 10 .mu.M H2O2 reduced their capicity to proliferate. After a 1 h exposure to 100 .mu.M H2O2 the cells were damaged to such extent that none survived. In the presence of lactoperoxidase and thiocyanate the cells were protected from the toxic effects of H2O2 as disclosed by their capacity to proliferate and exclude trypan blue. The rate of acid production of the cells in the presence of glucose was inhibited by the combination lactoperoxidase-thiocyanate-H2O2.