MEDIATION OF THE LOCALIZATION OF POLYMORPHONUCLEAR LEUKOCYTES IN GLOMERULI DURING THE AUTOLOGOUS PHASE OF NEPHROTOXIC NEPHRITIS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 35  (3) , 350-355
Abstract
A passive model of the autologous phase of nephrotoxic nephritis (NTN) in rabbits was developed to study the events at the initiation of this stage of the disease. I.v. injection of sheep anti-rabbit glomerular basement membrane antiserum was followed 48 h later by 125I trace labeled rabbit anti-sheep Ig[immunoglobulin]G. Animals were killed 3 h after the 2nd antibody injection. Experiments were undertaken to investigate whether or not a reaction between the passive antibody Fc piece and polymorphonuclear leukocyte (PMN) Fc receptor occurred, causing the localization of PMN in glomeruli in the autologous phase of NTN. Such a reaction is apparently an important, but not exclusive, factor in glomerular PMN localization. The complement independence of the localization was confirmed. It appeared that PMN-dependent intraglomerular fibrin deposition was mediated exclusively by a reaction between antibody Fc piece and PMN Fc receptor.