Evidence for histidine in the triethyltin-binding site of rat haemoglobin
- 1 January 1969
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 111 (2) , 129-137
- https://doi.org/10.1042/bj1110129
Abstract
One molecule of rat haemoglobin binds two molecules of triethyltin. The binding sites are located on the globin and there is co-operativity between the sites such that the intrinsic affinity constant at pH8·0 increases from 3·5×105m−1 for the binding of the first triethyltin molecule to 5·0×105m−1 for the binding of the second. Evidence is presented, from pH studies and the kinetics of inhibition due to photo-oxidation, that each binding site contains two histidine residues.Keywords
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