The Preparative Separation of Synthetic Peptides on Reversed-Phase Silica Packed in Radially Compressed Flexible-Walled Columns.+

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Abstract
The rapid and efficient separation of multigram amounts of unprotected synthetic peptides on octadecylsilica, packed in flexible walled polyethylene columns, is described. The mobile phase used was 0.05% trifluoroacetic acid with methanol as the organic modifier. An advantage of this eluant system was that the salt free peptide could be isolated simply by lyophilisation of the sample after chromatography. The following peptides were purified with this system: glycylgly-

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