Histochemical expression of lectin binding in papillomas.

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Abstract
The lectin-binding activity in papillomas arising from the skin, oral cavity, nasal, paranasal, and pharyngeal mucosa was examined by the use of the horseradish peroxidase (HRP) -conjugated lectin method. Keratinized epithelia in the skin and hyperkeratinized papillomas showed a marked regional or zonal distribution in binding of the lectins used; on the contrary, non-keratinized epithelia such as pseudo-stratified columnar epithelia had no zonal lectin distribution. The hyperortho-keratinized layer gave negative binding for the various lectins; the hyperpara-keratinized layer, slightly positive the granular cell layer containing keratohyalin granules and the upper spinous layer, the most prominent binding; and the basal cell layer, mostly negative. These lectin-binding patterns in the homologous epithelium were similar to that of keratinized papillomas. Cytochemical localization of such lectin binding was limited to intercellular materials and cellular surface in keratinized squamous epithelium and pseudo-stratified columnar epithelium. PA Con A-HRP staining was positive in granular materials located in the spinous layer cells, corresponding to glycogen-positive cells. Malignant or dysplastic cells in papillomas lesions were reduced in lectin binding and irregular in their distribution pattern.