The Distribution and Characteristics of Nitrate Reductase and Glutamate Dehydrogenase in the Maize Seedling
- 1 September 1973
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 52 (3) , 191-196
- https://doi.org/10.1104/pp.52.3.191
Abstract
In a study on 3-day maize (Zea mays) seedlings, grown on nitrate, requirements were established for the maximum extraction and optimum stabilization of nitrate reductase in vitro. With the primary root, 5 mm cysteine were required in the extraction medium, but for the scutellum, which has a high level of endogenous thiol, the use of additional thiol resulted in a reduced yield of a more labile enzyme. Activity of the root and scutella nitrate reductase was obtained with either NADH or NADPH, but that of the root enzyme with NADPH was only demonstrated in the absence of phosphate.Keywords
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