LOCALIZATION BY IMMUNOFLUORESCENCE OF GAMMA-GLOBULIN ALLOTYPES IN LYMPH NODE CELLS OF HOMOZYGOUS + HETEROZYGOUS RABBITS

  • 1 January 1964
    • journal article
    • research article
    • Vol. 7  (3) , 273-+
Abstract
The cellular production of 2 rabbit gamma-globulin allotypic specificities, A4 and A5, determined by allelic genes was investigated by the fluorescent antibody method. The 7S gamma-globulin fractions of precipitating antisera were conjugated to fluorescein isothiocyanate and to lissamine rhoda-mine B sulphonyl chloride. Frozen sections of lymph nodes from 18 rabbits, A4-A4 and A5-A5 homozygotes and A4-A5 heterozygotes, were studied after exposure to the fluorescent antibody conjugates. The conjugates, each specific for antigenic determinants of 7S gamma-globulin, reacted specifically with the cytoplasm of plasma cells and intrinsic cells of the germinal centres. The rabbit anti-A4 conjugate reacted only with lymph node cells of A4-A4 and A4-A5 rabbits; the rabbit anti-A5 conjugates reacted only with cells of A5-A5 and A4-A5 rabbits; the horse anti-rabbit gamma-globulin conjugates reacted with cells of all 3 genotypes. By a variety of techniques, identical cellular localization of the 2 allotypes, A4 and A5, was found in the A4-A5 heterozygotes. Less than 1% of the cells in any heterozygous lymph node section contained one allotype without the other.

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