Slow Migrating Proteinase Inhibitors in Human Urine

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Abstract
By a sensitive electrophoretic technique for the detection of proteinase inhibitors 3 slowly migrating proteinase inhibitors (SMPI) were discovered in some samples of pathological urine. SMPI 1 migrated in the .beta.2-zone whereas SMPI 2 and SMPI 3 appeared in the anodal and cathodal .gamma.-zone, respectively. Only SMPI 1 and 2 were examined in detail. These were found to inhibit tryptic and elastolytic digestion, but not chymotryptic or plasminolytic digestion of casein. Immunological investigations revealed no similarity to normally occurring proteinase inhibitors in serum and urine. The SMPI from one sample of urine were partially purified by DEAE-Sephadex ion exchange chromatography, followed by gel filtration on Sephacryl superfine 200. This procedure did not separate the 2 inhibitors. The molecular masses were estimated to be 25,000 Da [dalton] by gel filtration, and 23,000-26,500 Da by SDS polyacrylamide gel electrophoresis.

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