A New Technique in Paper Chromatography.

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Abstract
A continuous developing technique for descending paper chromatography uses a thick pad of cellulose tissue fastened to the foot of the column. The development is continued until the fastest moving component reaches the pad. The technique is described for amino acids. With tert.-amyl alcohol development for 2-3 wks., leucine, isoleucine, phenylalanine, methionine, valine, tryptophan, tyrosine and proline are completely separated from protein hydrolysates as individual spots. Alcohols cause less decomposition than collidine, isobutyric acid, pyridine, etc.