BASE-EXCISION REPAIR IN CARROT CELLS - PARTIAL-PURIFICATION AND CHARACTERIZATION OF URACIL-DNA GLYCOSYLASE AND APURINIC APYRIMIDINIC ENDODEOXYRIBONUCLEASE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 124  (3) , 435-440
Abstract
Uracil-DNA glycosylase and apurinic/apyrimidinic (AP) endoDNase were purified from cultured carrot cells. The 2 enzymes, separated by affinity chromatography on Sepharose-poly(rU), have properties similar to those of the homologous bacterial and mammalian enzymes. The action of AP endoDNase on (dA)230.cntdot.(dT,dU)230 partially depyrimidinated by uracil-DNA glycosylase suggests that these 2 enzymes might act successively to initiate the repair of uracil-containing DNA.