Cholesterol metabolism. 2. Cholesterol metabolism in the rat

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Abstract
Rats were fed basal diets containing 1.6% cholesterol and 16.6% added fat (cooking fat, butter, or olive oil) and cholesterol containing diets low in added fat. The fecal lipids were fractionated and on all diets fed there was an increase in excretion of acids, this being greatest with the fat-containing diets. There was a loss of unsaponifiable matter, and hence of cholesterol, on constructing a balance sheet and this loss was of the same order as the increase in the light petroleum-insoluble fecal acids. From the light petroleum-insoluble fraction of the fecal acids the derivative of an optically active acid of probably empirical formula C27H46O4 was isolated.
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