PROTEIN - CARBOHYDRATE INTERACTION .11. A STUDY OF TURBIDITY AS IT RELATES TO CONCANAVALIN A-GLYCAN INTERACTION

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  • 1 January 1968
    • journal article
    • research article
    • Vol. 14  (2) , 165-+
Abstract
A comparison of the behavior of the concanavalin [a globulin from Jack bean which agglutinates erythrocytes] A-polysac-charide and antibody-antigen systems with regard to various param-eters of turbidity was conducted. In common with antibody-antigen systems, the reaction of concanavalin A with polysaccharides shows that the ratio which yields the highest rate of development of turbidity is in the moderate concanavalin A excess region; a series of apparent equivalence curves are yielded by turbidimetry which differ from the equivalence curves obtained by the quantitative precipitin method; and a decrease in the rate of turbidity development occurs in the presence of hapten inhibitor. The degree of inhibition of the maximum constant turbidity caused by hapten inhibitors is identical to the degree of inhibition as determined by the amount of concanavalin A which precipitated. The quantitative estimation of inhibition (as determined turbidimetrically) at any time during the formation of turbidity gives results which are directly proportional to those obtained by the quantitative precipitin method.