Kinetics of expression of multiply spliced RNA in early human immunodeficiency virus type 1 infection of lymphocytes and monocytes.
- 1 June 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (11) , 5011-5015
- https://doi.org/10.1073/pnas.88.11.5011
Abstract
The genome of human immunodeficiency virus type 1 (HIV-1) encodes at least six proteins involved in regulation as well as the structural proteins Gag, Pol, and Env. The interplay of the various regulators generates early and late transcriptional phases in the HIV-1 life cycle; the earliest RNA is enriched in subgenomic species, and the genomic transcript appears at the later stage of infection. We investigated the nature of the mRNAs expressed in the early stages of infection when the 2 kilobase subgenomic species predominate. RNA was analyzed in the early phase of a one-step growth cycle of HIV-1 infection in T-lymphoid and monocytic cell lines by using PCR amplification of in vitro-synthesized viral cDNAs. In both cell lines, expression of Tat-, Rev-, and Nef-specific messages appeared simultaneously and could be detected within 8-12 hr of infection but in different amounts with a predominance of Nef-specific message. The Env-specific message could be detected as early as the Rev-specific message, indicating that expression of at least small amounts of the singly spliced message could occur before the accumulation of Rev.This publication has 23 references indexed in Scilit:
- Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latencyCell, 1990
- Immunodeficiency virus rev trans-activator modulates the expression of the viral regulatory genesNature, 1988
- Site-Directed Mutagenesis of Two Trans-Regulatory Genes ( tat -III, trs ) of HIV-1Science, 1988
- Generation of Protein Isoform Diversity by Alternative Splicing: Mechanistic and Biological ImplicationsAnnual Review of Cell Biology, 1987
- Mutational analysis of the human immunodeficiency virus: the orf-B region down-regulates virus replication.Proceedings of the National Academy of Sciences, 1987
- HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNACell, 1986
- Replicative and Cytopathic Potential of HTLV-III/LAV with sor Gene DeletionsScience, 1986
- Trans -Activator Gene of Human T-Lymphotropic Virus Type III (HTLV-III)Science, 1985
- Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirusNature, 1985
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985