Biologic Characterization of Human Gonadotropins Obtained from the Urine of Men, Postmenopausal Women and Eunuchs

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Abstract
HPG extracts obtained from the urine of men, postmenopausal women and eunuchs, processed by the kaolin-acetone procedure, were tested simultaneously in 4 bioassay systems. The end-points used measured total gonadotropic activity, follicle-stimulating (FSH) activity and lute.nizing (LH) activity. All assays were valid with regard to the statistical tests applied. In particular, there was no evidence of significant slope differences in any of the assays. The biologic activites of HPG from men and from postmenopausal women were similar, as judged by Gaddum's index of discrimination. The ratio of gonadotropic potency expressed as specific activity per milligram or specific activity per liter of urine remained unchanged, indicating that the FSH: LH ratio of the two preparations was the same. The biologic activity of eunuch HPG was not similar to that of HPG from men and from postmenopausal women, as evidenced by the indices of discrimination. The HPG from urine of eunuchs contained less LH per milligram than that of HPG from men and from postmenopausal women. However, per liter of urine, HPG from eunuchs contained more FSH than LH as compared with urinary HPG from men and from postmenopausal women. The FSH:LH ratio of HPG from eunuchs was about twice that of HPG from men or from postmenopausal women.