GRAM REACTION AND TETRAZOLIUM STAINING
- 1 February 1962
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 8 (1) , 99-103
- https://doi.org/10.1139/m62-013
Abstract
A simple method for differentiation of bacteria parallel to Gram staining is described, using vital staining with blue tetrazolium, decolorizing the preparation with a 1:1 mixture of acetone – ethylene tetrachloride for 4 hours, and counterstaining it with carbol fuchsin. Gram-negative bacteria are decolorized while the Gram-positive ones (except B. cereus) retain their formazan. Similar results were obtained by decolorizing vitally stained bacteria with organic solvents by chromatography.Keywords
This publication has 4 references indexed in Scilit:
- Effect of Tetrazolium Salts on Selected Bacterial Species.Experimental Biology and Medicine, 1960
- OBSERVATIONS ON THE USE OF TETRAZOLIUM SALTS IN THE VITAL STAINING OF BACTERIACanadian Journal of Microbiology, 1959
- Semiquantitative Evaluation of the Gram ReactionScience, 1958
- OBSERVATIONS ON THE REDUCTION AND QUANTITATION OF NEOTETRAZOLIUMJournal of Histochemistry & Cytochemistry, 1957