Effect of Tetrazolium Salts on Selected Bacterial Species.

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Abstract
Gram-negative bacteria are more resistant to the inhibitory effects of 5 tetrazolium compounds [triphenyl tetrazolium chloride (TTC), iodo-nitrotetrazolium (INT), neotetrazolium (NT), blue tetrazolium (BT), nitro-blue tetrazolium (NBT)] than are gram-positive organisms. Of the gram-negative organisms studied, Shigella sonnei was the least resistant and Proteus vulgaris the most resistant. With the exception of Staphylococcus aureus, which was unaffected by TTC, the gram-positive organisms were inhibited at tetrazolium concentrations ranging from less than 2 up to 64 [mu]g/ml. Ditetrazolium salts were more lethal than monotetrazolium salts. The order of decreasing inhibitory effectiveness for both gram-positive and gram-negative organisms was NBT, NT, BT, INT and TTC.