Studies onBacillus thuringiensis H-14 strains isolated in Egypt?III. Selection of media for ?-endotoxin production
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in World Journal of Microbiology and Biotechnology
- Vol. 6 (3) , 313-317
- https://doi.org/10.1007/bf01201303
Abstract
Several medium ingredients locally available in Egypt were investigated for their ability to support δ-endotoxin production by two mosquito-toxic strains ofBacillus thuringiensis H-14 usingCulex pipiens for the bioassay of δ-endotoxin. Soya beans, black-eyed beans, common peas and lentils supported good production of toxin whereas peanuts, fodder yeast, cheese whey and corn steep liquor gave only low amounts of toxin. Molasses as the sole carbon source at 2 and 3% (w/v) with soya (or Proflo) as the sole nitrogen source at 3% gave the best yields of toxin for strain M1 and S128 respectively. On a étudié plusieurs ingrédients de milieu de culture, disponibles en Egypte pour leur capacité de favoriser la production d'endotoxine δ par deux souches deBacillus thurigiensis H-14, toxiques pour les moustiques, en utilisantCulex pipiens pour l'essaiin vivo d'endotoxine δ. Les fèves de soja, les haricots à taches noires, les pois communs et les lentilles favorisent la production de toxine, tandis que les arachides, la levure fourragère, le petit lait et la liqueur de trempage de maïs ne produisent que de faibles quantités de toxines. La mélasse comme seule source de carbone à 2% (p/v) avec la soja (ou le Proflo) comme seule source d'azote de 2 à 3% donnent les meilleurs rendements en toxine.Keywords
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