Microscopic and flow cytophotometric analysis of parasitemia in cultures ofPlasmodium falciparum vitally stained with Hoechst 33342 ?application to studies of antimalarial agents

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Abstract
Conditions for rapid vital staining ofPlasmodium falciparum infected human erythrocytes were 1 μg/ml of the dye Hoechst 33342 for 15 min in the standard culture medium at 37° C. Fixed and stained cultures were analyzed by fluorescence microscopy and flow cytophotometry. The usefulness of this type of analysis for in vitro studies of antimalarial agents was demonstrated using three such agents—cyclosporin A, chloroquine, and pyrimethamine.