Factors affecting the heat resistance of Bacillus stearothermophilus spores

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Abstract
Summary. With increase in sporulation temperature (50‐55‐60°C) there was a significant increase in the heat resistance at 115°C of Bacillus stearothermophilus spores produced at these temperatures. the heat resistance of spores was not related to the manganese sulphate concentration in the sporulation medium except at high concentrations (1000 ppm). the heat resistance of spores in McIlvaine's citric acid‐phosphate or Sörensen's phosphate buffers was greatest at pH 7‐0: survivor curves showed an exponential death rate with no heat activation response. Spores were significantly more resistant when heated in water as compared with the various buffers.