PRESENCE OF 1,25-DIHYDROXYVITAMIN-D3 RECEPTORS IN ESTABLISHED HUMAN CANCER CELL-LINES IN CULTURE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (3) , 1116-1119
Abstract
A range of established human cancer cell lines were examined for the presence of receptors for 1,25-(OH)2D3 [1,25-dihydroxyvitamin D3] and calcitonin. Thirty-three cancer cell lines were examined. 1,25-DR [1,25-(OH)2D3 receptor] was present in 23 lines, while calcitonin receptors were not detected in any of them. The 1,25-DR from several cell lines sedimented at about 3.5S in sucrose density gradients, had the appropriate specificity for vitamin D metabolites, had Kds of 0.8 to 2.2 .times. 10-11 M, and had receptor concentrations of 12-99 fmol/mg protein. Ten malignant melanoma and 9 colonic carcinoma lines constituted the largest groups of carcinoma cell lines, and 7 and 8, respectively, of these were 1,25-DR positive. The high frequency of 1,25-DR positivity in the cultured colonic carcinoma cells is quite different from the low frequency of 1,25-DR in primary colonic carcinomas. Both of 2 cell lines derived from patients who had had both bone metastases and malignant hypercalcemia were 1,25-DR positive. These various cell lines may provide useful models for the examination of 1,25-(OH)2D3 action in vitro.