Lung Transit of 111Indium‐Labelled Granulocytes

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Abstract
The early in vivo distribution of 111In-labeled granulocytes, recorded by dynamic imaging using a gamma camera and computer, varied according to the separation and labeling technique. Following i.v. bolus injection, 4 kinetic patterns could be identified: (A) rapid transit through the pulmonary vasculature; (B) delayed transit through the lung with clearance by .apprx. 30 min; (C) complete retentin by the lung, for up to 10 min, followed by slow release over a period of 1-2 h and (D) delayed transit through the lung with a time course of 30 min but with subsequent heavy liver uptake. Granulocytes labeled with 111In-tropolonate and maintained in plasma throughout the labeling procedure, whether injected as a pure (separated by plasma-enriched density gradient centrifugation) or crude (separated by differential centrifugation) preparation. displayed type A kinetics, though to most closely represent in the normal behavior of granulocytes. Crude cells labeled in saline with 111In-acetylacetonate displayed type B kinetics. Pure cells isolated on Percoll-saline and labeled in saline with 111In-acetylacetonate displayed type C kinetics, thought to represent granulocyte stimulation and/or damage, or type D kinetics, thought to represent severe damage. The importance is stressed of labeling granulocytes for kinetic studies with a technique that results in minimal alteration of cell behavior.