Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking threonine 104 phosphorylation
Open Access
- 1 March 2003
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 84 (3) , 727-732
- https://doi.org/10.1099/vir.0.18972-0
Abstract
Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)-associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr104 in TMV MP. The MP-specific PKs with apparent molecular masses of about 45–50 kDa and 38 kDa were revealed by gel PK assays. Two types of mutations were introduced in TMV MP gene of wild-type TMV U1 genome to substitute Thr104 by neutral Ala or by negatively charged Asp. Mutation of Thr104 to Ala did not affect the size of necrotic lesions induced by the mutant virus in Nicotiana tabacum Xanthi nc. plants. Conversely, mutation of Thr to Asp mimicking Thr104 phosphorylation strongly inhibited cell-to-cell movement. The possible role of Thr104 phosphorylation in TMV MP function is discussed.Keywords
This publication has 22 references indexed in Scilit:
- Intramolecular Complementing Mutations in Tobacco Mosaic Virus Movement Protein Confirm a Role for Microtubule Association in Viral RNA TransportJournal of Virology, 2002
- Phosphorylation of viral movement proteins – regulation of cell-to-cell traffickingTrends in Microbiology, 2001
- Nucleic Acid Transport in Plant-Microbe Interactions: The Molecules That Walk Through the WallsAnnual Review of Microbiology, 2000
- Changing Patterns of Localization of the Tobacco Mosaic Virus Movement Protein and Replicase to the Endoplasmic Reticulum and Microtubules during InfectionPlant Cell, 1998
- Cell-to-Cell and Long-Distance Transport of Viruses in PlantsPlant Cell, 1996
- Multiple serine phosphorylation sites on the 30 kDa TMV cell‐to‐cell movement protein synthesized in tobacco protoplastsThe Plant Journal, 1995
- Molecular Cloning, Functional Expression in Escherichia coli, and Characterization of Multiple Mitogen‐Activated‐Protein Kinases from TobaccoEuropean Journal of Biochemistry, 1995
- Phosphorylation of tobacco mosaic virus cell-to-cell movement protein by a developmentally regulated plant cell wall-associated protein kinase.Genes & Development, 1993
- In vivo phosphorylation of the 30‐kDa protein of tobacco mosaic virusFEBS Letters, 1992
- The Tobacco Mosaic Virus 30K Movement Protein in Transgenic Tobacco Plants is Localized to PlasmodesmataJournal of General Virology, 1991