Structural determination of the active site of a sweet protein A 1H NMR investigation of pMNEI
Open Access
- 21 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 310 (1) , 27-30
- https://doi.org/10.1016/0014-5793(92)81138-c
Abstract
PMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the β‐sheet of pMNEI that contains a likely ‘sweet finger’: the loop connecting the β‐strands from residue 59 to residue 78, corresponding to segment 16–35 of the A chain of monellin. The detailed three‐dimensional structure of the loop (Tyr66‐Ala67‐Ser68‐Asp69), determined from several interresidue and intraresidue NOEs and subsequent energy minimization, shows that the side chains or Tyr66 and Asp69 fit our model of the sweet receptor in a manner very similar to that of the side chains of Phe and Asp or aspartame.Keywords
This publication has 19 references indexed in Scilit:
- Analytical treatment of spin diffusion in a three-spin (AMX and ABX) system in the long-correlation-time (ω0gtc, ⪢ 1) limit, for estimation of internuclear distances in macromoleculesJournal of Magnetic Resonance (1969), 1990
- Conformation-activity relationship of sweet molecules. Comparison of aspartame and naphthimidazolesulfonic acidsJournal of Medicinal Chemistry, 1990
- An alternative method for distance evaluation from NOESY spectraJournal of Magnetic Resonance (1969), 1988
- A procedure for preparing models of receptor sitesJournal of Chemical Education, 1986
- Practical aspects of two-dimensional transverse NOE spectroscopyJournal of Magnetic Resonance (1969), 1985
- Soft agonist receptor interactions: Theoretical and experimental simulation of the active site of the receptor of sweet moleculesInternational Journal of Quantum Chemistry, 1984
- Structure-sweetness relationship of L-aspartyl dipeptide analogs. A receptor site topologyJournal of Medicinal Chemistry, 1981
- Three-dimensional mapping of the bitter taste receptor siteChemical Senses, 1979
- Three-dimensional mapping of the sweet taste receptor siteJournal of Medicinal Chemistry, 1978
- Chemoreception of sweet-tasting dipeptide esters: A third binding siteFood Chemistry, 1978