Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α
- 3 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 297 (1-2) , 175-178
- https://doi.org/10.1016/0014-5793(92)80354-j
Abstract
Prostaglandin F2α (PGF2α), a mitogen for resting Swiss 3T3 cells, rapidly stimulates phosphorylation of an 80 kDa protein (80 K). 1‐Oleoyl‐2‐acetylglycerol (OAG) and 12‐O‐tetradecanoyl phorbol‐13‐acetate (TPA) both protein kinase C (PKC) activators, also elicit 80 K phosphorylation. In contrast PGE1, PGE2 or PGF2β, which are non‐mitogenic in these cells, had tittle or no action on this event. However PGE1 and PGE2 potentiate the PGF2α proliferative effect but do not enhance its action on 80 K phosphorylation. These results suggest that PGF2α mitogenic induction involves PKC signalling pathway activation while its enhancement by PGE1 or PGE2 occurs through a different mechanism(s).Keywords
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